|
KMID : 0363620090300040079
|
|
Journal of Korean Oriental Medicine
2009 Volume.30 No. 4 p.79 ~ p.92
|
|
|
Neuroprotective Effect of PD-1 Extract in MPTP-lesioned Mouse Model of Parkinson’s Disease
|
|
Lee Jung-Wook
Jung Hye-Mi
Seo Un-Kyo
|
|
|
Abstract
|
|
|
Objectives: The aim of the present study was to explore the neuroprotective effect and the possible mechanism of the PD-1 extracts on 1-methyl-4-phenyl-1,2,3,6-tetrahydrophridine (MPTP)-lesioned C57BL/6 mouse model of Parkinson’s disease (PD).
Methods: The mice were supplemented (or not) with 50 or 100 mg/kg/day of PD-1 for 2 weeks, after which MPTP was injected intraperitoneally. We observed that daily administration of PD-1 prevented MPTP-induced depletion of striatal DA, and maintained striatal and nigral tyrosine hydroxylase (TH) protein levels.
Results: Our results demonstrated that mice treated with PD-1 prior to MPTP administration showed more abundant TH-immunopositive (TH-ir) fibers and neurons than mice given only MPTP, indicating that PD-1 protects dopaminergic striatal fibers and nigral neurons from MPTP insults. Possible neuroprotective effect of PD-1 was further studied by the detection of antiapoptotic protein (bcl-2) and proapoptotic protein (Bax). In this assay, MPTP elevated the Bax protein and decreased the bcl-2 protein, while these expressions were prevented by PD-1 pre-treatment.
Conclusions: The present results suggest that PD-1 is able to protect dopaminergic neurons from MPTP-induced neuronal injury with anti-apoptotic activity being one of the possible mechanisms.
|
|
|
KEYWORD
|
|
|
PD-1, Parkinson disease, 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydrophridine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|